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Webinar

Rethinking Oligonucleotide LC-MS Analysis: Optimizing Workflows for Quantification and Qualification

ET
60 Minutes

Oligonucleotide LC-MS workflows are often dictated by stringent project requirements, necessitating complex sample preparation to meet sensitivity demands and/or extensive chromatographic separation for ample baseline resolution. As interest in therapeutic oligos increases, teams need accessible workflows that can support quantitative and qualitative performance while enabling deeper assessment of related species, impurities, and modifications. 

In this webinar, scientists from QPS will share an oligonucleotide bioanalysis case study showing how sample extraction and LC workflow decisions shaped method performance on the SCIEX ZenoTOF 8600 system.

Building on the QPS study, SCIEX showcase complementary, qualitative oligo mapping and impurity analysis using IP-RP LC-MS/MS. The discussion will demonstrate how accurate mass measurement, sequence analysis, chromatographic separation and automated data analysis can support mass analysis, impurity assessment, and sequence confirmation in addition to quantitative measurement.

By the end of this webinar, attendees will be able to:

  • Evaluate key oligonucleotide bioanalysis workflow to assess recovery, quantitation and study-specific challenges
  • Apply extraction, LC conditions and MS optimization strategies to improve quantitative results
  • Assess where high-resolution MS and EAD can extend bioanalysis beyond quantitation to support broader characterization

Speakers

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Jessie Le, Ph.D.

Research Scientist, Bioanalysis
QPS LLC

Jessie Le is a bioanalytical scientist specializing in high-resolution mass spectrometry and method development for complex biological molecules. She earned her Ph.D. in Chemistry from UCLA under Professor Joseph Loo, where she developed native top-down mass spectrometry approaches for characterizing membrane proteins and biomolecular assemblies. Currently as a Research Scientist at QPS Holdings, her focus is to develop and validate high-throughput workflows for oligonucleotide therapeutics, complex biotherapeutics, and biomarkers using high-resolution time-of-flight mass spectrometry.

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Haichuan Liu

Staff Scientist
SCIEX

Haichuan Liu is a Staff Scientist at SCIEX. Haichuan specializes in characterization of protein and oligonucleotide-based therapeutics using orthogonal LC-MS workflows and alternative fragmentation techniques. Haichuan has over 30 years of experience in various fields of mass spectrometry and is an author of nearly 60 peer-reviewed articles. He has also published numerous biopharma application notes and presented his scientific work at many conferences.

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